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\u8be5\u533a\u57df\u4e3b\u8981\u8fdb\u884c\u7684\u64cd\u4f5c\u4e3a\u4e34\u5e8a\u6807\u672c\u7684\u4fdd\u5b58\u3001\u6838\u9178(RNA\u3001DNA)\u63d0\u53d6\u3001\u8d2e\u5b58\u53ca\u5176\u52a0\u5165\u81f3\u6269\u589e\u53cd\u5e94\u7ba1\u548c\u6d4b\u5b9aRNA\u65f6cDNA\u7684\u5408\u6210\u3002<\/p>\n\n\n\n \u672c\u533a\u7684\u538b\u529b\u68af\u5ea6\u8981\u6c42\u4e3a\uff1a\u76f8\u5bf9\u4e8e\u90bb\u8fd1\u533a\u57df\u4e3a\u6b63\u538b\uff0c\u4ee5\u907f\u514d\u4ece\u90bb\u8fd1\u533a\u8fdb\u5165\u672c\u533a\u7684\u6c14\u6eb6\u80f6\u6c61\u67d3\u3002\u53e6\u5916\uff0c\u7531\u4e8e\u5728\u64cd\u4f5c\u4e2d\u53ef\u80fd\u4f1a\u53d1\u751f\u6c14\u6eb6\u80f6\u6240\u81f4\u7684\u6c61\u67d3\uff0c\u6240\u4ee5\u5e94\u907f\u514d\u5728\u672c\u533a\u5185\u4e0d\u5fc5\u8981\u7684\u8d70\u52a8\u3002<\/p>\n\n\n\n 3.\u6269\u589e\u53cd\u5e94\u6df7\u5408\u7269\u914d\u5236\u548c\u6269\u589e\u533a<\/strong><\/p>\n\n\n\n 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PCR\u5b9e\u9a8c\u5ba4\u8bbe\u8ba1\u7684\u6838\u5fc3\u95ee\u9898\u662f\u5982\u4f55\u907f\u514d\u6c61\u67d3\u3002\u5728\u5b9e\u9645\u5de5\u4f5c\u4e2d\uff0c\u5e38\u89c1\u7684\u6709\u4ee5\u4e0b\u51e0\u79cd\u6c61\u67d3\u7c7b\u578b\uff1a\u6269\u589e\u4ea7\u7269\u7684\u6c61\u67d3\uff1b\u5929\u7136\u57fa\u56e0\u7ec4DNA\u7684\u6c61\u67d3\uff1b\u8bd5\u5242\u7684\u6c61\u67d3\u4ee5\u53ca\u6807\u672c\u95f4\u7684\u6c61\u67d3\u3002\u7531\u4e8e\u4e00\u65e6\u53d1\u751f\u6c61\u67d3\uff0c\u5b9e\u9a8c\u5c31\u5fc5\u987b\u505c\u6b62\uff0c\u76f4\u5230\u627e\u5230\u6c61\u67d3\u6e90\u4e3a\u6b62\uff0c\u800c\u4e14\u5b9e\u9a8c\u7ed3\u679c\u5fc5\u987b\u4f5c\u5e9f\uff0c\u9700\u91cd\u65b0\u8fdb\u884c\u5b9e\u9a8c\u3002\u6240\u4ee5\u53d1\u751f\u6c61\u67d3\u540e\u518d\u56f4\u7ed5\u5b9e\u9a8c\u5ba4\u6765\u5bfb\u627e\u6c61\u67d3\u6e90\u4e0d\u4f46\u8017\u65f6\u800c\u4e14\u7e41\u7410\uff0c\u6d6a\u8d39\u4eba\u529b\u7269\u529b\u3002\u56e0\u6b64\u8981\u907f\u514d\u6c61\u67d3\uff0c\u9996\u5148\u5e94\u662f\u9884\u9632\uff0c\u800c\u4e0d\u662f\u6392\u9664\u3002<\/p>\n\n\n\n 1.\u5de5\u4f5c\u533a\u57df\u7684\u4e25\u683c\u5212\u5206<\/strong><\/p>\n\n\n\n \uff081\uff09\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u8bbe\u7f6e\u5408\u7406\u3002<\/p>\n\n\n\n \uff082\uff09\u5404\u4e2a\u5b9e\u9a8c\u533a\u57df\u8981\u6709\u660e\u663e\u7684\u6807\u8bb0\uff08\u5982\u9192\u76ee\u7684\u95e8\u724c\u6216\u4e0d\u540c\u7684\u5730\u9762\u989c\u8272\u7b49\uff09\uff0c\u4ee5\u907f\u514d\u5404\u4e2a\u4e0d\u540c\u5b9e\u9a8c\u533a\u57df\u8bbe\u5907\u7269\u54c1\u3001\u8bd5\u5242\u7b49\u53d1\u751f\u6df7\u6dc6\u3002<\/p>\n\n\n\n 2.\u5408\u7406\u7684\u7cfb\u7edf\u8bbe\u7f6e<\/strong><\/p>\n\n\n\n \uff081\uff09\u5408\u7406\u7684\u7a7a\u8c03\u901a\u98ce\u7cfb\u7edf\u8bbe\u7f6e\uff0c\u5c3d\u91cf\u91c7\u7528\u5168\u9001\u5168\u6392\u7684\u7a7a\u8c03\u7cfb\u7edf\u3002<\/p>\n\n\n\n \uff082\uff09\u4e25\u683c\u7684\u6c14\u6d41\u538b\u529b\u63a7\u5236\uff0c\u4fdd\u8bc1\u4e0d\u540c\u7684\u5b9e\u9a8c\u533a\u5185\u4e0d\u540c\u7684\u538b\u529b\u8981\u6c42\u3002<\/p>\n\n\n\n 3.\u89c4\u8303\u5730\u64cd\u4f5c<\/strong><\/p>\n\n\n\n \uff081\uff09\u4e34\u5e8a\u57fa\u56e0\u6269\u589e\u68c0\u9a8c\u5b9e\u9a8c\u5ba4\u7684\u6280\u672f\u4eba\u5458\u5fc5\u987b\u8fdb\u884c\u4e0a\u5c97\u57f9\u8bad\uff0c\u7ecf\u57f9\u8bad\u5408\u683c\u540e\u624d\u80fd\u4ece\u4e8b\u4e34\u5e8a\u57fa\u56e0\u6269\u589e\u68c0\u9a8c\u7684\u5de5\u4f5c\u3002<\/p>\n\n\n\n \uff082\uff09\u5728\u5b9e\u9a8c\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\uff0c\u64cd\u4f5c\u8005\u5fc5\u987b\u6234\u624b\u5957\uff0c\u5e76\u7ecf\u5e38\u66f4\u6362\u3002\u6b64\u5916\uff0c\u64cd\u4f5c\u4e2d\u4f7f\u7528\u4e00\u6b21\u6027\u5e3d\u5b50\u4e5f\u662f\u4e00\u4e2a\u6709\u6548\u5730\u9632\u6b62\u6c61\u67d3\u7684\u63aa\u65bd\u3002<\/p>\n\n\n\n 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